| 质粒名称: | pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000465 |
| 产品用途: | to express mBaoJin(mouse) in mouse through Cre dependent |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mBaoJin(mouse) |
| 物种来源: | mouse |
| 密码子优化: | 老鼠表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Bright and stable monomeric green fluorescent protein derived from StayGold
Zhang H., Leslov G.D., Subach O.M., Zhang W., Kuzmicheva T.P.P., Vlaskina A.V.V., Samygina V.R.R., Chen L., Ye X., Nikolaeva A.Y.Y., Gabdulkhakov A., Papadaki S., Qin W., Borshchevskiy V., Perfilov M.M., Gavrikov A.S.S., Drobizhev M., Mishin A.S.S., Piatkevich K.D.
Nat. Methods 2024 214 21, 657–665 (2024)
|
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pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse)载体质粒基本信息
pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse)质粒图谱载体图谱和pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse)载体序列质粒序列多克隆位点信息
载体图谱.png)
pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse)质粒载体简介
pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse)质粒序列载体序列
LOCUS pAAV-CAG-DIO-mBaoJin(codon-optimized for mouse) 5755 bp DNA circular SYN 03-APR-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-CAG-DIO-mBaoJin(codon optimized for mouse)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5755)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Apr 29, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..5755
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 1950..2562
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(1..861)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(862..966)
/gene="bla"
/label=AmpR promoter
rep_origin complement(992..1447)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 1338..1358
/label=WKG-465-F
misc_feature 1519..1648
/label=AAV2 ITR
misc_feature 1709..1949
/label=CAG
misc_feature 1950..2562
/label=CAG
enhancer 1950..1957
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1959..2236
/label=chicken beta-actin promoter
primer_bind 2610..2636
/label=209-WKG-1044-F3
protein_bind 2654..2687
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
protein_bind 2750..2783
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS complement(2811..3512)
/codon_start=1
/label=mBaoJin(codon-optimized for mouse)
/translation="MVSKGEEENMASTPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
KYVCTSGKLPMSWAALGTTFGYGMKYYTKYPSGLKNWFREVMPGGFTYDRHIQYKGDGS
IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMNVSLPNEVPQIPRDDGVECPVTL
LYPLLSDKSKYVEAHQYTICKPLHNQPAPDVPYHWIRKQYTQSKDDAEERDHICQSETL
EAHLKGMDELYK"
regulatory 3509..3518
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
protein_bind complement(3538..3571)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are
compatible with each other, but incompatible with loxP or
loxN sites (Lee and Saito, 1988)."
protein_bind complement(3642..3675)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
misc_feature 3717..4305
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
misc_feature 3853..3858
/label=SDS
CDS complement(4188..4199)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 4348..4469
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 4815..5755
/label=ColE1
/note="ColE1"
rep_origin complement(4997..5585)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
961 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
1021 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
1081 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
1141 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
1201 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
1261 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
1321 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
1381 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
1441 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
1501 tcttcgctat tacgccagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
1561 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
1621 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
1681 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
1741 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
1801 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
1861 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
1921 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
1981 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
2041 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
2101 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
2161 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
2221 aagcgcgcgg cgggcgggag tcgctgcgac gctgccttcg ccccgtgccc cgctccgccg
2281 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
2341 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
2401 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
2461 gctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
2521 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
2581 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
2641 gaattggatc cccataactt cgtataaagt atcctatacg aagttatatc aaaataggaa
2701 gaccaatgct tcaccatcga cccgaattgc caagcatcac catcgaccca taacttcgta
2761 taatgtatgc tatacgaagt tatactaggg atccccttac ttgtacatta cttgtaaagt
2821 tcgtccatcc ctttgagatg tgcttcaaga gtttctgatt ggcagatatg atcccgttct
2881 tcggcgtcgt cttttgattg ggtatattgt ttgcggatcc agtggtaggg aacatctgga
2941 gctggctgat tgtgcagggg tttgcagatg gtatattggt gggcttcgac gtacttagac
3001 ttatcactca gaagagggta gaggagagtc actggacact ctacaccgtc atccctagga
3061 atttgaggga cttcatttgg cagtgatacg ttcatgtctc cggtgagtac gggtgaattc
3121 tctttaaaat cttgaccggt aaactccaca atattatgat aggtgccgtt tttcataaaa
3181 tgctggtgct tagcatgtat gctgccgtcg cctttatact gtatgtgtct gtcataggtg
3241 aaccctccag gcataacttc cctaaaccaa tttttcagtc cacttgggta tttagtatag
3301 tatttcatcc cgtacccgaa tgtagtcccg agtgcagccc agctcatagg aagttttcca
3361 gaggtacaaa cgtactttcc tttatgagag ccttcgtggg agtttccctc tccctcacct
3421 tccactgtga atgatttgcc gttgattgtt cccttgagtt gaaacttaaa gggggtggaa
3481 gccatattct cttcctctcc tttgctgacc atggtggctt aggtaccgaa ttctagtata
3541 acttcgtata ggatacttta tacgaagtta tcattgggat tcttcctatt ttgatccaag
3601 catcaccatc gaccctctag tccagatctc accatcgacc cataacttcg tatagcatac
3661 attatacgaa gttatgtccc tcgaagaggt tcgaattcga tatcaagctt atcgataatc
3721 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
3781 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
3841 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
3901 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
3961 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
4021 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
4081 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
4141 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
4201 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
4261 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
4321 ccgggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
4381 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
4441 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
4501 aggttcaggg ggagatgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
4561 aaatcgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
4621 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
4681 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
4741 gtgagcgagc gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
4801 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
4861 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
4921 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
4981 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
5041 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
5101 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5161 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5221 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5281 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5341 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5401 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
5461 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5521 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5581 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
5641 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
5701 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacag
//
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